GINS2 affects activity/differentiation, apoptosis and proliferation of osteoblast and osteoclast in steroid-induced osteonecrosis of the femoral head by regulating P53/GADD45A signaling pathway

To investigate the function and mechanism of GINS complex subunit 2 (GINS2) in osteoblast osteoclast steroid-induced osteonecrosis femoral head (SINFH). Clinical specimens were collected from 40 cases SINFH patients control patients. Then, mouse model was built. Immediately, osteoblasts osteoclasts induced differented pre-osteoblast cell line MC3T3-E1 mononuclear macrophage RAW264.7 mice, respectively. The qRT-PCR assay used for detecting expressions GINS2, p53 growth arrest DNA damage 45A (GADD45A). cytoactive, proliferation apoptosis mice measured by tartaric acid phosphatase staining, CCK-8 Annexin V-FITC/PI dual GINS2 up-regulated while p53/GADD45A down-regulated tissues mice. GADD45A which presented opposite trends formations. decreased activities increased activities, inhibited promoted via pathway. affects activity/differentiation, regulating signaling